ylmH [2019-08-15 13:05:01]

unknown

locus: BSU_15410

pI: 7.04

mw: 28.92 kDa

protein length: 257 aa

gene length: 774 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.7|Proteins of unknown function]

Gene

Coordinates: 1,611,654 → 1,612,427

The protein

Protein family

• [SW|S4 RNA-binding domain] superfamily (according to http://www.ebi.ac.uk/interpro/entry/IPR036986 Interpro)

[SW|Domains]

• [SW|S4 RNA-binding domain] (aa 181 ... 251) (according to UniProt)

Structure

• [PDB|2FPH] (from Streptococcus pneumoniae, N-terminal domain, corresponds to aa 4 ... 158, 32% identity)

Expression and Regulation

Operons

• genes: [gene|ECCE99438DBFC52DA7236CB4F6486DD004CADF73|ylmD]-[gene|31789A170CB624BF8210C915F40007F802F5C81B|ylmE]-[gene|DB09F1C36257F511A84A083967A25A9D46744D14|sepF]-[gene|2B2D285D5A8F160808A21DCF07BDB924365970C5|ylmG]-[gene|BD5ACF930DD63E258D71569326752C9A1D7B9324|ylmH]
  description: [Pubmed|16420366]

  regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: repression, [Pubmed|14651647], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]

  regulation

  • repressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647]

Biological materials

Mutant

• MGNA-B364 (ylmH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1363 NBRP B. subtilis, Japan]
• BKE15410 (Δ[gene|BD5ACF930DD63E258D71569326752C9A1D7B9324|ylmH]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15410 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGTCTTTCCCTTCTATG, downstream forward: _UP4_TAGTCGGTTTTTCAGTTTTT
• BKK15410 (Δ[gene|BD5ACF930DD63E258D71569326752C9A1D7B9324|ylmH]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15410 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGTCTTTCCCTTCTATG, downstream forward: _UP4_TAGTCGGTTTTTCAGTTTTT

References

• 14651647,16420366